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  • © 2018

ENZYMES: Catalysis, Kinetics and Mechanisms

Authors:

  • This book caters the need of the students studying Biochemistry, Enzymology or Industrial Microbiology as part of their Biotechnology/ Life Sciences course
  • The book synchronizes two broad mechanistic facets of enzymology namely, the chemical and the kinetic
  • It discusses on how to begin experiments with an enzyme and subsequently analyze the data collected
  • Highly illustrated with figures and tables

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Table of contents (40 chapters)

  1. Front Matter

    Pages i-xxiii
  2. Enzyme Catalysis – A Perspective

    1. Front Matter

      Pages 1-1
    2. Enzymes: Their Place in Biology

      • N. S. Punekar
      Pages 3-4
    3. Enzymes: Historical Aspects

      • N. S. Punekar
      Pages 5-13
    4. On Enzyme Nomenclature and Classification

      • N. S. Punekar
      Pages 33-41
    5. Hallmarks of an Enzyme Catalyst

      • N. S. Punekar
      Pages 43-51
    6. Origins of Enzyme Catalytic Power

      • N. S. Punekar
      Pages 53-70
    7. Which Enzyme Uses What Tricks?

      • N. S. Punekar
      Pages 71-74
  3. Enzyme Kinetic Practice and Measurements

    1. Front Matter

      Pages 83-83
    2. Chemical Kinetics: Fundamentals

      • N. S. Punekar
      Pages 85-96
    3. Concepts of Equilibrium and Steady State

      • N. S. Punekar
      Pages 97-106
    4. ES Complex and Pre-steady-state Kinetics

      • N. S. Punekar
      Pages 107-114
    5. Principles of Enzyme Assays

      • N. S. Punekar
      Pages 115-129
    6. Good Kinetic Practices

      • N. S. Punekar
      Pages 131-142
    7. Henri–Michaelis–Menten Equation

      • N. S. Punekar
      Pages 155-176
    8. More Complex Rate Expressions

      • N. S. Punekar
      Pages 177-191
    9. Enzyme Kinetic Data: Collection and Analysis

      • N. S. Punekar
      Pages 193-211

About this book

This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to plan enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers.

The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject.

Authors and Affiliations

  • Department of Biosciences & Bioengineering, Indian Institute of Technology Bombay, Mumbai, India

    N.S. Punekar

About the author

Dr. N. S. Punekar is currently working as a Professor at prestigious Indian Institute of Technology (IIT) Bombay, Mumbai, India. He obtained his PhD from the coveted Indian Institute of Sciences, Bangalore, India in the year 1984 and subsequently worked as Post-doctoral fellow at University of Wisconsin, Madison, USA till 1988. He joined IIT Bombay as Assistant Professor in 1988 and subsequently got elevated to the rank of Professor in 2001.

Dr. Punekar’s major research interest lies in Microbial Biochemistry and Molecular Enzymology; Microbial Metabolic Regulation, Understanding Metabolism through biochemical and recombinant DNA techniques, Fungal Molecular Genetics and its Applications to Metabolic Engineering. Dr. Punekar has published around 50 papers in peer reviewed reputed journals and more importantly has 4 patents to his credit. He has been an excellent teacher of Enzymology and Industrial Microbiology which is evident by the fact that he has received the “Excellence in Teaching Award” at IIT Bombay in the years 2000 and 2012. He possesses more than two decades of experience of teaching Enzymology at IIT Bombay.

He is associated with various societies and committees of Government of India as an expert member. He is also in editorial board of journals like Indian Journal of Biochemistry and Biophysics, Indian Journal of Biotechnology since and Indian Journal of Experimental Biology, etc.

Bibliographic Information

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access